Food Testing, GMO testing, Animal Species detection, Food Authenticity testing, Microbial Identification testing, Rapid Pathogen Detection Food

Molecular Solutions for Food

Authenticity, GMO testing and Microbial Monitoring laboratory services concern Food & Beverages, Feed & Crops, Water & Wastewater, Environmental & Industrial sectors. QACS Molecular testing services for food have a wide accreditation spectrum. For environmental & food molecular testing, contact us, email info@qacs.gr or give a call at + 30 210 29 34745

Authenticity

We monitor and verify preparations quality by applying sensitive molecular techniques to detect food adulteration. Authenticity of the species origin in food and feed products is crucial for consumer safety- sensitive group needs require for specific analysis (HALAL, Vegan).

Animal Species detection with Real Time PCR detects Breeds e.g. Sheep, Goat, Bovine, Horse, Chicken, Turkey and other animal species in food products is conducted in routine. The LOD is 0,1% and TAT 3-5 working days.

DNA Barcoding, genetic identity (Barcoding) is a powerful tool in favor of botanical, fish or animal authenticity. With specific molecular tools, a small part of the DNA of an organism is multiplied via PCR and its sequence is analyzed in a Genetic Sequencer. The resulting sequence is compared with others in validated sequence databases like B.O.L.D (Barcode of Life Data System) and the species name is assigned. By analyzing and “reading” this piece of DNA containing the organism’s genetic identity, an indisputable “DNA certificate” of the species of the organism is obtained. In this way, the product or raw material acquires a “seal of authenticity” and the producer or distributor is protected from counterfeiting (replacement of part or all of it by another product of lower value) and the product acquires a higher added value. The TAT is 10-15 working days.

Organisms profile (NGS analysis), in biological mixtures like sausages, cheeses or pies, Next Generation sequencing (NGS) is used to identify any organism that is contained at the species level qualitatively and quantitatively. As a result, the proportion of every organism is given. This technique has revealed in the past extensive food in composed foods. TAT 30 working days.

GMO

GMO testing is crucial for producers who want to verify the marketability of their product, fulfill labeling requirements and comply with EU Regulation (EC) No. 1830/2003 and EU Regulation (EC) No. 619/2011. Qualitative and Quantitative GMO analysis assist you reach testing & industry needs.  QACS examines genetic modification presence on seeds, flour, feed and food.

We offer a wide range of accredited genetic modifications tests for soyacorn and cotton and other plant species.

DNA isolation and subsequently detection of specific genetic elements

P35S

TNOS and pFMV

Elements indicate genetic modification via Real Time PCR (LOD 0,045%). The TAT is 3-5 working days.

Quantification analysis follows to quantify the extend of genetic modification  If the LOD is below 0,9%, the declaration is not necessary as it is attributed to unintended contaminations.  TAT is 1-2 working days.

Upon request common genetic modifications are identified and quantified. In this case a “event specific” analysis is performed.

  • GM soy in Europe is GTS 40-3-2 soy
  • Roundup Ready Soya

Microbial Monitoring

At the Molecular biology lab, it is possible to identify microorganisms at the species level using DNA technology. The DNA identification method is globally recognized as the most reliable for the classification of microorganisms. In addition to the micro-organism species name, information about the ecology, classification, growth conditions and possible pathogenesis are provided.

Identification is achieved in 7 hours, starting from a single colony.

Identification is achieved in 8 hours, starting from a single colony. Sequence is multiplied via PCR and finally its sequence is determined with Sanger Sequencing. Resulting sequence is compared to assign the species name. Along with the species name, in the analysis report, a description of the microorganism is included to facilitate targeted corrective actions.

Mold identification with classical microbiological method takes weeks. With  molecular testing identification is achieved in 8 hours, starting from a single colony.

The resulting sequence is compared with validated sequence databases and the species name is assigned. Furthermore, along with the species name, in the analysis report, a description of the microorganism is included to facilitate targeted corrective actions.

Methodology

The DNA identification method is globally recognized as the most reliable for the classification of microorganisms. DNA identification techniques present a clear reliability advantage over other methods for example APIs that are metabolic, MALTI TOF  or other immunochemical methods.

DNA fragments reveal information about the microorganism’s species (bacteria/yeast/fungi). Method used PCR and consequently analyzed in a Genetic Analyzer. Finally, the sequence is compared with gene databases.

In addition to the micro-organism species name, information on the ecology, classification, growth conditions and possible pathogenesis are provided. Upon request we may provide scientific instructions and solutions to resolve the causes of microbial contamination, through investigation of the microbiological origin and analysis of the deeper causes.

The technique includes DNA isolation and detection of specific genetic elements indicating genetic modification via Real Time PCR. If detected, a quantification analysis follows to quantify the extend of genetic modification. As a third step, if requested, the genetic modification could be identified.

Worldwide pathogenic bacteria are of growing public health concern. We perform fast (24h) and accurate pathogen detection via Real Time PCR to ensure the product’s safety.

Real Time PCR analysis for the sensitive detection of pork DNA (HALAL) and other animal species in food products is conducted in routine.