Regulatory compliance and consumer safety should be ensured for all cosmetics & personal care products therefore, manufacturers are responsible for identifying harmful ingredients and proceed to cosmetic testing.
With respect of animal rights and regulatory requirements QACS employs alternatives to toxicity testing based on Replacing, Reducing & Refining e.g. In-Vitro studies. We assess the efficacy and toxicology of cosmetics, household, personal care, industrial and chemical products using cells on reconstructed tissues.
In-Vitro studies follow validated and in house methods to ensure cosmetic safety & product marketability. Sensitization, Toxicity, Efficacy and Tolerance evaluation methods are undertaken by our professionals.
→ We use in vitro testing primarily in regulatory safety testing and chemical product evaluation. We further conduct in vitro testing to select and rank chemicals during development of new chemicals, products and in toxicology research.
This in vitro hazard assessment assay can differentiate materials that are ocular non-irritants from materials that are ocular irritants. As part of the protocol, we apply the test substance on a non-keratinized epithelium, prepared from normal human keratinocytes. The ability to expose the tissue topically is essential to model the same kind of progressive injury expected in vivo. It also allows both solid and liquid test materials to be applied directly to the tissue.
We determine cell viability after the application via MTT assay. For the final determination of cell viability we use the ELISA photometer. A substance is classified as irritating if the mean cell viability is of a minimum 2 under test tissues below 60% of the negative control.
This in vitro test is based on the effective time at which a material (applied neat) causes a 50% reduction in tissue viability (ET-50). Based on the ET-50%, the test article is classified into 1 of 4 classifications, ranging from non-irritating, very mild, mild, moderate to severe/extreme. These correspond to groupings of Draize rabbit eye test scores (MMAS).
Eye irritation tests have been:
- Validated by ECVAM for substances and mixtures.
- Certified as good laboratory practice (GLP).
We use the Bovine Corneal Opacity and Permeability test method (BCOP) as an alternative to the Draize rabbit eye test to identify:
- Chemicals Inducing Serious Eye Damage
- Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage (OECD 437)
We conduct the test on isolated corneas from the eyes of cattle.
We use the Reconstructed Human Epidermis Test Method (OECD 439). We apply the test substance in different conditions on reconstructed human epidermis (RhE). We determine cell viability after the application via MTT assay. For the final determination of cell viability we use the ELISA photometer. A substance is classified as irritating if the mean cell viability of a minimum of 3 under test tissues is below 50% of the negative control.
The in vitro skin irritation test has:
- Been validated by ECVAM as a reference method.
- Received regulatory acceptance for substances and mixtures.
- Been certified as good laboratory practice (GLP).
We use the Reconstructed Human Epidermis Test Method (OECD 431). We expose the test substance topically to a reconstructed human epidermis (RhE) model and then conduct a cell viability test. We measure cell viability by dehydrogenase conversion of MTT present in cell mitochondria, into a blue formazan salt that we quantitatively measure photometrically after extraction from tissues. Skin corrosivity potential of the test materials is classified according to the remaining cell viability obtained after a 3-minute or 1-hour exposure to the test chemical.
→ validated by ECVAM as a reference method.
We expose the test substance topically to an organotypic human airway tissue model (EpiAirway) for 3 hours. We then measure tissue viability IC75 (the dose required to reduce the EpiAirway culture viability to 75% of vehicle treated tissues).
We measure cell viability by dehydrogenase conversion of MTT present in cell mitochondria, into a blue formazan salt that we quantitatively measure photometrically after extraction from tissues.
We predict the inhalation irritation potential through the reduction of the average viability of 2 tissues exposed to the test substance in comparison to the average viability of 2 negative controls (treated with sterile deionized water).