In Vitro cosmetic testing
With respect to animal rights and regulatory requirements QACS employs alternatives to toxicity testing based on Replacing, Reducing & Refining e.g. In-Vitro studies. Cosmetic regulatory compliance and consumer safety should be ensured for all personal care products therefore, manufacturers are responsible for identifying harmful ingredients and proceeding to cosmetic testing. At QACS we assess the efficacy and toxicology of cosmetics, household, personal care, industrial and chemical products using cells on reconstructed tissues.
In-Vitro analysis follows validated and in house methods to ensure cosmetic safety & product marketability. Sensitization, Toxicity, Efficacy and Tolerance evaluation methods are undertaken by our professionals.
→ We use in vitro testing primarily in regulatory safety testing and chemical product evaluation. We further conduct in vitro testing to select and rank chemicals during development of new chemicals, products and in toxicology research.
This method can be used to predict the ocular irritation potential of chemicals, substances and mixtures. Specifically, the intended use of the test is to identify chemicals not requiring classification and labelling as substances that can cause serious eye damage or irritation.
The method was assessed in a series of validation studies and is regulatory approved under ECVAM, can be used with essentially all types of chemicals/substances and can be used to identify substances not requiring classification for serious eye damage/eye irritation. The test was accepted in 2015 as Test Guideline 492.
The test is based on a 3D tissue model, of reconstructed kind of human cornea-like epithelium which closely mimics various properties of the human corneal epithelium. Relative tissue viability after exposure to a test substance is measured with a cytotoxicity assay with the outcome being used as a proxy for eye irritation or serious eye damage.
We use the Bovine Corneal Opacity and Permeability test method (BCOP) as an alternative to the Draize rabbit eye test to identify:
- Chemicals Inducing Serious Eye Damage
- Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage (OECD 437)
We conduct the test on isolated corneas from the eyes of cattle.
The EpiDerm Skin Irritation Test (SIT) method can be used to predict the skin irritation potential of chemicals/substances.
This method has been previously validated in a series of validation studies and has now received regulatory approval as a full replacement method for equivalent in vivo test methods for skin irritation potential.
This test method is based on a one hour topical exposure of a chemical to the reconstructed human epidermis model followed by a test for cell viability. Based on the cell viability outcome, a chemical is classed as an irritant if ≤50% are viable after the exposure time in comparison to a negative control (exposure to water). Conversely a chemical is classed as a non-irritant when >50% are viable after exposure.
The EpiDerm Skin Corrosion Test (SCT) method can be used to predict the skin corrosion potential of chemicals/substances.
The test has been scientifically validated and granted regulatory approval for the identification and classification of the corrosive potential of chemicals.
This Test addresses the human health endpoint skin corrosion. It makes use of reconstructed human epidermis (RhE) (obtained from human derived non-transformed epidermal keratinocytes) which closely mimics the histological, morphological, biochemical and physiological properties of the upper parts of the human skin, i.e. the epidermis. The RhE-based skin corrosion test methods have shown to be predictive of in vivo skin corrosion effects assessed in rabbits according to the OECD guideline 404. The prediction model of the EpiDerm™ SCT skin corrosion test methods is associated with the UN GHS classification system.
The EpiAirway 3D human tissue model is routinely utilized for a range of applications including safety & risk assessment. More specifically, the EpiAirway Toxicity Testing Protocol can analyse the potential irritant effects to the respiratory tract, using human 3D reconstructed airway tissues.